| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
A simple ATAC-seq protocol for population epigenetics
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| Autores: |
De Carvalho Augusto, Ronaldo
Rey, Oliver
Cosseau, Céline
Chaparro, Cristian
Vidal-dupiol, Jeremie
Allienne, Jean-françois
Duval, David
Pinaud, Silvain
Tönges, Sina
Andriantsoa, Ranja
Luquet, Emilien
Aubret, Fabien
Dia Sow, Mamadou
David, Patrice
Thomson, Vicki
Joly, Dominique
Gomes Lima, Mariana
Federico, Déborah
Danchin, Etienne
Minoda, Aki
Grunau, Christoph
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| Data: |
2020
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| Ano: |
2020
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| Palavras-chave: |
Epigenetics
Epigenomics
ATAC-seq
Daphnia pulex
Schistosoma mansoni
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| Resumo: |
We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size (~10,000 cells), and it requires little molecular biology handling expertise.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00681/79353/81859.pdf
DOI:10.12688/wellcomeopenres.15552.2
https://archimer.ifremer.fr/doc/00681/79353/
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| Editor: |
F1000 Research Ltd
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| Formato: |
application/pdf
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| Fonte: |
Wellcome Open Research (2398-502X) (F1000 Research Ltd), 2020 , Vol. 5 , P. 121 (24p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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